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Characterization of a novel human endogenous retrovirus, HERV-H/F, expressed in human leukemia cell lines

Author:
  • S Patzke
  • Mats Lindeskog
  • E Munthe
  • HC Aasheim
Publishing year: 2002
Language: English
Pages: 164-173
Publication/Series: Virology
Volume: 303
Issue: 1
Document type: Journal article
Publisher: Elsevier

Abstract english

We have identified and characterized a human endogenous retrovirus (HERV) gag transcript in the human pre-B cell leukemia line Reh. The transcript was found to be a splice product of a structurally intact HERV element located on chromosome 6q13. Its primer binding site is complementary to phenylalanine (F) tRNA, common for the HERV-F family, but the overall genome sequence is closely related to the HERV-H family. The retroviral sequence was therefore designated HERV-H/F The HERV element shows a distinct mRNA expression pattern among hematopoietic cancer cell lines with expression in some leukemia-derived cell lines of B-lymphoid and myeloid origin. No expression was observed in normal human tissues, indicating a cancer-specific expression pattern. The 5' long terminal repeat (LTR) was tested for promoter activity in HERV-H/F expressing and nonexpressing cell lines. The cell specificity of the LTR-mediated reporter gene expression did not conclusively correlate with endogenous virus expression, indicating that the transcription regulation of this gene is not alone dependent on cell-specific activity of transcription factors. (C) 2002 Elsevier Science (USA).

Keywords

  • Microbiology in the medical area
  • LTR
  • leukemia
  • HERV
  • endogenous retrovirus
  • expression

Other

Published
  • ISSN: 1096-0341
E-mail: mats [dot] lindeskog [at] nateko [dot] lu [dot] se

Postdoctoral fellow

Dept of Physical Geography and Ecosystem Science

+46 46 222 42 57

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Department of Physical Geography and Ecosystem Science
Lund University
Sölvegatan 12
S-223 62 Lund
Sweden

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