Anna Maria Jönsson
Head of department
The use of light and electron microscopy to assess the impact of ozone on Norway spruce needles
Author
Summary, in English
Light (LM) and transmission electron (TEM) microscopy were used to study previously specified ozone symptoms in the foliage of Norway spruce. The three youngest green needle generations from twenty mature trees in two stands on sites of different soil fertility at Asa, southern Sweden, were sampled in 1999. The critical dose of ozone, expressed as AOT40, was 6362 ppb.h. LM showed ozone-specific symptoms: decreased chloroplast size with electron dense stroma advancing gradually from the outer to the inner cell layers, being most severe in the needle side facing the sky. The symptoms were expressed as ozone syndrome indices at the needle generation, tree and stand levels. The index had higher values at the low fertility site. TEM was used to confirm the LM results. The study shows that LM can be used for diagnosis of the impact of ozone on conifers in the field.
Department/s
- Dept of Physical Geography and Ecosystem Science
- Department of Biology
Publishing year
2004
Language
English
Pages
441-453
Publication/Series
Environmental Pollution
Volume
127
Issue
3
Document type
Journal article
Publisher
Elsevier
Topic
- Ecology
Status
Published
ISBN/ISSN/Other
- ISSN: 0269-7491